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KMID : 0903519950380030212
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 3 p.212 ~ p.217
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Biochemical Changes in Brassica Seedlings Due to Cold Acclimation Treatment
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Abstract
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This study was aimed for determining the biochemical mechanism of cold tolerance in crops and for searching the biochemical genetic marker related with cold tolerance by the analysis of isozyme pattern. We investigated various biochemical changes induced by the long-term cold acclimation in cold sensitive rape (B. napus) and in cold tolerant $quot;Sandongchae$quot;(B. campestris) seedlings. The cold shock after long-term cold acclimation to B. napus and B. campesris greatly increased the activities of peroxidase 157% and 50% in root fraction and, 201% and 205% in hypocotyl, respectively. Simultaneously, the activity of superoxide dismutase was largely increased in hypocotyl fraction, too. Protein contents of hypocotyl fractions in B. napus and B. campestris were also increased by 11.4% and 57.8%, respectively. The band of pI 6.4 among peroxidase isozymes newly biosynthesized during long-term cold acclimation was emerged in the hypocotyl fraction of cold tolerant it. campestris as well as in the root of both species. From above and previous results, we presented a model of interconversions of molecular oxygen species due to the cold injury and biochemically inferred the mechanism of cold tolerance in crops.
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KEYWORD
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